Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7229441 | Biosensors and Bioelectronics | 2018 | 23 Pages |
Abstract
In this work, Ru(bpy)32+ encapsulated in metal-organic frameworks (MOFs) UiO-67 (Ru(bpy)32+/UiO-67) as luminophor was easily prepared and firstly applied in constructing an electrochemiluminescence (ECL) immunosensor to efficiently estimate diethylstilbestrol (DES). The competitive method-based ECL immunosensor platform was fabricated by amino-silicon dioxide which possesses large surface area. The poriness of UiO-67 was splendid so that Ru(bpy)32+ could be easily encapsulated. Ru(bpy)32+/UiO-67 with excellent ECL luminescence signal existed large specific surface area for easily labeled with antibodies. DES competed with bovine serum albumin-diethylstilbestrol (BSA-DES) for binding to antibody-specific sites in the constructed immunosensor. However DES was micromolecule, which was easier to bond to antibodies than BSA-DES. The ECL signal was gradually decreases with the increase of the concentration of DES. Under optimal conditions, the proposed immunosensor exhibited a wide linear range from 0.01â¯pgâ¯mLâ1 to 50â¯ngâ¯mLâ1 with a low detetion limit of 3.27 fg mLâ1 (S/Nâ¯=â¯3). The novel fabricated immunosensor with interference immunity and high stability may cause an attractive approach for the other targets determination.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Xue Dong, Guanhui Zhao, Li Liu, Xuan Li, Qin Wei, Wei Cao,