Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7229469 | Biosensors and Bioelectronics | 2018 | 20 Pages |
Abstract
Two new inorganic-organic hybrid materials based on heteropolyoxometalates (POMs): (C4H10N)6[P2Mo18O62]·4H2O (P2Mo18) and (C6H8NO)4[H2P2W18O62]·6H2O (P2W18) are reported as mediators for electron transfer between FAD-dependent glucose dehydrogenase (FAD-GDH) and a multiwalled carbon nanotube (MWCNT) matrix for glucose biofuel cell and biosensor applications. These polyoxometalates were chosen due to their promising redox behavior in a potential range for mediated electron transfer with the glucose oxidizing enzyme, FAD-GDH. P2Mo18 and P2W18 were immobilized on 1-pyrenemethylamine (PMA) functionalized MWCNT deposits. After immobilization of FAD-GDH, the P2W18-modified MWCNT electrode demonstrated mediated electron transfer and provided a catalytic current density of 0.34â¯mAâ¯cmâ2 at 0.2â¯V vs SCE with an open circuit potential (OCP) of â0.08â¯V vs SCE. A 10-fold increase in catalytic current to 4.7â¯mAâ¯cmâ2 at 0.2â¯V vs SCE and a slightly lower OCP of â0.10â¯V vs SCE was observed for an equivalent electrode modified with P2Mo18.The apparent superiority of P2Mo18 is related, at least in part, to its improved incorporation in the MWCNT matrix compared to P2W18. Both POM-modified bioanodes showed exceptional stabilities with 45% of their initial performances remaining after 15 days. The mediated electron transfer capacities of the POMs were also evaluated in a glucose sensor setup and showed very satisfying performances for glucose detection, including a sensitivity of 0.198â¯mAâ¯molâ¯Lâ1 cmâ2, a satisfying linear range between 1â¯mmolâ¯Lâ1 and 20â¯mmolâ¯Lâ1, and good reproducibility for the P2Mo18 electrode.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Feriel Boussema, Andrew J. Gross, Fatma Hmida, Brahim Ayed, Hatem Majdoub, Serge Cosnier, Abderrazak Maaref, Michael Holzinger,