Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7229559 | Biosensors and Bioelectronics | 2018 | 5 Pages |
Abstract
There has been an urgent need to quickly screen and isolate patients with viral infections from patients with similar symptoms at point-of-care. In this study, we introduce a new microfluidic method for detection of various viruses using rolling circle amplification (RCA) of pathogens on the surface of thousands of microbeads packed in microchannels. When a targeted pathogen meets the corresponding particular template, the DNAs are rapidly amplified into a specific dumbbell shape through the RCA process, forming a DNA hydrogel and blocking the flow path formed between the beads. Due to the significant increase in reaction surface area, the detection time was shortened to less than 15â¯min and the detection limit of various pathogens has been reached to 0.1â¯pM. By injecting the stained liquid, the existence of the target pathogens in a sample fluid can be determined with the naked eye. Furthermore, by integrating multi-channel design, simultaneous phenotyping of various infective pathogens (i.e., Ebola, Middle East respiratory syndrome (MERS), and others) in biological specimens can be performed at a point-of-care.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Wonhwi Na, Dongwoo Nam, Hoyoon Lee, Sehyun Shin,