Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7229620 | Biosensors and Bioelectronics | 2018 | 7 Pages |
Abstract
MXene-Ti3C2, as a new class of two-dimensional (2D) transition metal carbides (or nitrides), has been synthesized by exfoliating pristine Ti3AlC2 phases with hydrofluoric acid. The SEM and XRD images show that the resultant MXene possesses a graphene-like 2D nanostructure. and the surface of MXene has been partially terminated with -OH, thus providing a favorable microenvironment for enzyme immobilization and retaining their bioactivity and stability. Considering the unique metallic conductivity, biocompatibility and good dispersion in aqueous phase, the as-prepared MXene was explored as a new matrix to immobilize tyrosinase (a model enzyme) for fabricating a mediator-free biosensor for ultrasensitive and rapid detection of phenol. The varying electrochemical measurements were used to investigate the electrochemical performance of MXene-based tyrosinase biosensors. The results revealed that the direct electron transfer between tyrosinase and electrode could be easily achieved via a surface-controlled electrochemical process. The fabricated MXene-based tyrosinase biosensors exhibited good analytical performance over a wide linear range from 0.05 to 15.5â¯Î¼molâ¯Lâ1, with a low detection limit of 12â¯nmolâ¯Lâ1 and a sensitivity of 414.4â¯mAâ¯Mâ1. The proposed biosensing approach also demonstrated good repeatability, reproducibility, long-term stability and high recovery for phenol detection in real water samples. With those excellent performances, MXene with graphene-like structure is proved to be a robust and versatile electrochemical biosensing platform for enzyme-based biosensors and biocatalysis, and has wide potential applications in biomedical detection and environmental analysis.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Lingxia Wu, Xianbo Lu, Dhanjai Dhanjai, Zhong-Shuai Wu, Yanfeng Dong, Xiaohui Wang, Shuanghao Zheng, Jiping Chen,