Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7229823 | Biosensors and Bioelectronics | 2018 | 16 Pages |
Abstract
An electrochemical biosensor was developed based on a steric hindrance hybridization assay to allow the highly sensitive detection of streptavidin. In the steric hindrance hybridization assay, the signaling strand DNA (sig-DNA) was labeled at the 3Ⲡend with CdSe quantum dots (QDs) and at the 5Ⲡend with biotin, and capturing strand DNA (the complementary strand of sig-DNA) was labeled at the 5Ⲡend with thiol. The steric hindrance effect generated by streptavidin which was bound with the signaling DNA strand. The streptavidin limited the ability of the sig-DNA to hybridize with the cap-DNA, which were linked on the surface of a gold electrode. Therefore, the concentration of streptavidin was detected indirectly based on the concentration of CdSe QDs on the electrode surface. The concentration of CdSe QDs on the electrode surface was detected by differential pulse anodic stripping voltammetry. Under optimal conditions, the streptavidin detection range using the as-prepared biosensor was 1.96 pg/mL to 1.96 µg/mL and the detection limit was 0.65 pg/mL. The experimental results showed that the electrochemical biosensor could detect streptavidin rapidly and accurately.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Yu-ping Wei, Xing-Pei Liu, Chang-jie Mao, He-Lin Niu, Ji-Ming Song, Bao-Kang Jin,