Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7230105 | Biosensors and Bioelectronics | 2016 | 8 Pages |
Abstract
DNA aptamers that can bind specific molecular targets have great potential as probes for microbial diagnostic applications. However, aptamers may change their conformation under different operating conditions, thus affecting their affinity and specificity towards the target molecules. In this study, a new integrated microfluidic system was developed that exploited the predictable change in conformation of a single universal influenza aptamer exposed to differing ion concentrations in order to detect multiple types of the influenza virus. Furthermore, the fluorescent-labeled universal aptamer used in this system could distinguish and detect three different influenza viruses (influenza A H1N1, H3N2, and influenza B) at the same time in 20Â min and therefore has great potential for point-of-care applications requiring rapid diagnosis of influenza viruses.
Keywords
Mg++ddH2OPBSPMTPDMSFAMMicrofluidicsinfBRT-PCRSELEXCa++Na+double-distilled waterAptamerRNAribonucleic acidInfaLOD یا Limit of detectionMagnetic beadslimit of detectionPhosphate-buffered salineHemagglutinationreverse transcriptase polymerase chain reactionInfluenza B virusInfluenza virusInfluenza A virusPolydimethylsiloxanecarboxyfluoresceinnegative controlSodium ionmagnesium ionpotassium ionCalcium ion
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Chih-Hung Wang, Chih-Peng Chang, Gwo-Bin Lee,