Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7230262 | Biosensors and Bioelectronics | 2016 | 32 Pages |
Abstract
A label-free immunosensor based on electrochemical impedance spectroscopy has been developed for the sensitive detection of a cardiac biomarker myoglobin (cMyo). Hydrothermally synthesized graphene quantum dots (GQDs) have been used as an immobilized template on screen printed electrodes for the construction of an impedimetric sensor platform. The GQDs-modified electrode was conjugated with highly specific anti-myoglobin antibodies to develop the desired immunosensor. The values of charge transfer resistance (Rct) were monitored as a function of varying antigen concentration. The Rct value of the immunosensor showed a linear increase (from 0.20 to 0.31 kΩ) in the range of 0.01-100 ng/mL cMyo. The specific detection of cMyo was also made in the presence of other competing proteins. The limit of detection for the proposed immunosensor was estimated as 0.01 ng/mL which is comparable to the standard ELISA techniques.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Satish K. Tuteja, Rui Chen, Manil Kukkar, Chung Kil Song, Ruchi Mutreja, Suman Singh, Ashok K. Paul, Haiwon Lee, Ki-Hyun Kim, Akash Deep, C. Raman Suri,