| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 7230285 | Biosensors and Bioelectronics | 2017 | 7 Pages |
Abstract
We report a new method for the detection of regional DNA methylation using base-dependent affinity interaction (i.e., adsorption) of DNA with graphene. Due to the strongest adsorption affinity of guanine bases towards graphene, bisulfite-treated guanine-enriched methylated DNA leads to a larger amount of the adsorbed DNA on the graphene-modified electrodes in comparison to the adenine-enriched unmethylated DNA. The level of the methylation is quantified by monitoring the differential pulse voltammetric current as a function of the adsorbed DNA. The assay is sensitive to distinguish methylated and unmethylated DNA sequences at single CpG resolution by differentiating changes in DNA methylation as low as 5%. Furthermore, this method has been used to detect methylation levels in a collection of DNA samples taken from oesophageal cancer tissues.
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Authors
Md. Hakimul Haque, Vinod Gopalan, Sharda Yadav, Md Nazmul Islam, Ehsan Eftekhari, Qin Li, Laura G. Carrascosa, Nam-Trung Nguyen, Alfred K. Lam, Muhammad J.A. Shiddiky,