Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7230389 | Biosensors and Bioelectronics | 2016 | 24 Pages |
Abstract
A label-free fiber optic biosensor based on a long period grating (LPG) and a basic optical interrogation scheme using off the shelf components is used for the detection of in-situ DNA hybridization. A new methodology is proposed for the determination of the spectral position of the LPG mode resonance. The experimental limit of detection obtained for the DNA was 62±2 nM and the limit of quantification was 209±7 nM. The sample specificity was experimentally demonstrated using DNA targets with different base mismatches relatively to the probe and was found that the system has a single base mismatch selectivity.
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Authors
Helena M.R. Gonçalves, Luis Moreira, Leonor Pereira, Pedro Jorge, Carlos Gouveia, Paula Martins-Lopes, José R.A. Fernandes,