Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7230425 | Biosensors and Bioelectronics | 2016 | 8 Pages |
Abstract
We present a novel ratiometric pH sensor design using water-soluble, dual-emission, Mn2+-doped quantum dots (Qdots) decorated with D-penicillamine (DPA-MnQdots). In contrast to more commonly used ratiometric pH sensors that rely on the coupling of two fluorophores, our design uses only a single emitter, which simplifies ratiometric sensing and broadens the applications of the sensor. Our single-emitter DPA-MnQdots exhibit two emission bands, at 510Â nm (green) and 610Â nm (red), which are, respectively, attributable to exciton recombination and emission of the Mn2+ dopants. The emission intensity ratio (I510/I610) of the DPA-MnQdots depends linearly on surrounding pH values within physiological conditions (from pH 4.5 to 8.5). Moreover, the biocompatible DPA-MnQdots were used for long-term monitoring of local pH values in HeLa cells.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Feby Wijaya Pratiwi, Chih-Hao Hsia, Chiung Wen Kuo, Shun-Min Yang, Yeu-Kuang Hwu, Peilin Chen,