Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7230436 | Biosensors and Bioelectronics | 2016 | 25 Pages |
Abstract
In pH 7.4 Na2HPO4-NaH2PO4 buffer solution containing the peptide probes for human chorionic gonadotropin (hCG), silver nanoparticles (AgNPs) were aggregated to big AgNPs clusters that exhibited very weak catalytic effect on the gold nanoparticle reaction of H2O2-HAuCl4. When hCG was present in the peptide probe solution, the AgNPs did not aggregate and it had strong catalytic effect on the gold nanoparticle reaction with a strong resonance Rayleigh scattering (RRS) peak at 370Â nm and a strong surface enhanced Raman scattering (SERS) peak at 1615Â cmâ1 in the presence of molecular probe of Victoria blue 4R (VB4R). With the increase of the hCG concentration, the catalysis enhanced due to the nanocatalyst of AgNPs increasing, and the RRS intensity increased at 370Â nm. The increased RRS intensity was linear to the hCG concentration in 0.05-10Â ng/mL, with a linear regression equation of ÎI370Â nm=409.8Â C +294. And the SERS intensity at 1615Â cmâ1 increased linearly with the hCG concentration in the range of 0.05-20Â ng/mL, with a linear regression equation of ÎI1615Â cm-1=142Â C+134. Based on this, two new methods of nanocatalytic SERS and RRS were proposed for the determination of trace hCG.
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Authors
Guiqing Wen, Xiaojing Liang, Qingye Liu, Aihui Liang, Zhiliang Jiang,