Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7230631 | Biosensors and Bioelectronics | 2016 | 7 Pages |
Abstract
A novel nanoparticle based biosensor for the fast and simple detection of DNA hybridization events is presented. The sensor utilizes hybridized DNA's charge transport properties, combining them with metallic nanoparticle networks that act as nano-gapped electrodes. The DNA hybridization events can be detected by a significant reduction in the sensor's resistance due to the conductive bridging offered by hybridized DNA. By modifying the nanoparticle surface coverage, which can be controlled experimentally being a function of deposition time, and the structural properties of the electrodes, an optimized biosensor for the in situ detection of DNA hybridization events is ultimately fabricated. The fabricated biosensor exhibits a wide response range, covering four orders of magnitude, a limit of detection of 1Â nM and can detect a single base pair mismatch between probe and complementary DNA.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Evangelos Skotadis, Konstantinos Voutyras, Marianneza Chatzipetrou, Georgios Tsekenis, Lampros Patsiouras, Leonidas Madianos, Stavros Chatzandroulis, Ioanna Zergioti, Dimitris Tsoukalas,