Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7230790 | Biosensors and Bioelectronics | 2016 | 21 Pages |
Abstract
A sandwich-type electrochemical immunosensor was developed for sensitive detection of carcinoembryonic antigen (CEA) by using ferroferric oxide@silica-amino groups (Fe3O4@SiO2-NH2) as carriers and gold nanoparticles-graphene oxide (GO-AuNPs) as platform. The Fe3O4@SiO2-NH2 surface was used as linked reagents for co-immobilization of ferrocenecarboxylic acid (Fc-COOH) and secondary anti-CEA (Ab2) to prepare the signal probe, and it also could hasten the decomposition of hydrogen peroxide (H2O2) to amplify signals. Differential pulse voltammetry (DPV) was successfully used to quantify CEA. Under the optimized conditions, the designed immunosensor shows an excellent analytical performance wide dynamic response range of CEA concentration from 0.001 ng mLâ1 to 80 ng mLâ1 with a relatively low detection limit of 0.0002 ng mLâ1 (S/N=3), and high specificity and good reproducibility. The proposed immunosensor was successfully used to determine CEA in spiked human serum samples.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Taotao Feng, Xiuwen Qiao, Haining Wang, Zhao Sun, Chenglin Hong,