Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7230799 | Biosensors and Bioelectronics | 2016 | 8 Pages |
Abstract
Novel fluorescent nitrogen-doped carbon dots (N,C-dots) were synthesized and assembled on aptamer modified gold nanoparticles (Aptamer/AuNPs) for the super sensitive detection of aflatoxin B1 (AFB1). Positively charged N,C-dots were synthesized by the hydrothermal treatment of pancreatin. The prepared N,C-dots were assembled on aptamer/AuNPs by electrostatic interactions. The fluorescence of the N,C-dots was efficiently quenched. When AFB1 was added to the assay solution, specific interactions between AFB1 and the aptamer caused release of the N,C-dots. The fluorescence of the N,C-dots recovered and the intensity increase could be used to calculate the amount of AFB1 added. The assay exhibits super-high sensitivity with a detection limit of 5Â pg/mL (16Â pM) and a wide range of linear response of 5Â pg/mL to 2.00Â ng/mL. A novel aptasensor is thus successfully constructed, it provides an efficient way for sensitive AFB1 sensing as well as a new technique for aptamer based novel sensor construction.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Bin Wang, Yanfen Chen, Yuanya Wu, Bo Weng, Yingshuai Liu, Zhisong Lu, Chang Ming Li, Cong Yu,