l-cysteine induced manganese porphyrin electrocatalytic amplification with 3D DNA-Au@Pt nanoparticles as nanocarriers for sensitive electrochemical aptasensor

We successfully applied the novel l-cysteine (RSH) induced manganese porphyrin (MnTPP) electrocatalytic amplification in electrochemical aptasensor for detection of thrombin (TB) with the 3D DNA-Au@Pt nanoparticles (DNA-Au@Pt NPs) as nanocarrier. A further discussion for the suggested mechanism of the reaction involved the MnTPP-catalyzed oxidation of thiols (RSH) to disulfides l-cystine (RSSR) was also discussed in detail in this work. In comparison with traditional H2O2 mediated MnTPP electrocatalytic amplification, the novel reaction with more stable catalytic substrate RSH possessed high catalytic amplification efficiency. In addition, the 3D DNA-Au@Pt NPs could provide abundant binding sites for immobilizing signal tags and enzymes. As a result, the electrochemical signal can be greatly enhanced by the RSH mediated MnTPP electrocatalytic reaction and 3D DNA-Au@Pt NPs. Under optimal conditions, the proposed aptasensor exhibited a wider linear range of 0.1 pM-100 nM with a more sensitive detection limit of 29 fM for TB detection.