Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7230843 | Biosensors and Bioelectronics | 2016 | 7 Pages |
Abstract
Rare earth-doped upconversion nanoparticles have promising potential in the field of pesticide detection because of their unique frequency upconverting capability and high detection sensitivity. This paper reports a novel aptamer-based nanosensor for acetamiprid detection using fluorescence resonance energy transfer (FRET) between NH2-NaYF4: Yb, Ho@SiO2 (UCNPs) and gold nanoparticles (GNPs). Herein, GNPs as acceptors efficiently quench the fluorescence of UCNPs and acetamiprid specifically interacts with acetamiprid binding aptamer (ABA), causing the conformation changes of ABA from random coil to hairpin structure. Accordingly, ABA no longer stabilizes the GNPs in salt solution, leading to the varying aggregation extent of GNPs. Thus, the fluorescence of UCNPs are proportionally recovered. Under the optimized conditions, the enhancement efficiency was observed to increase linearly with the concentration of acetamiprid from 50Â nM to 1000Â nM, resulting in a relatively low limit of 3.2Â nM. Additionally, the aptasensor demonstrated high selectivity to similar structure pesticides such as imidacloprid and chlorpyrifos, and further confirmed its application capacity in adulterated tea samples.
Related Topics
Physical Sciences and Engineering
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Authors
Weiwei Hu, Quansheng Chen, Huanhuan Li, Qin Ouyang, Jiewen Zhao,