Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7230909 | Biosensors and Bioelectronics | 2016 | 7 Pages |
Abstract
Based on specific aptamer binding properties, a strategy for adenosine, dopamine and 17β-estradiol detection was realised by employing Ru complex and quantum dots (QDs) as fluorescence probes. Ru complex, which could quench the fluorescence of QDs, preferred to bind with aptamer DNA and resulted in the fluorescence rise of QDs. When the aptamer DNA was incubated with the target first, it could not bind with Ru complex and the fluorescence of QDs was quenched. Under the optimal condition, the fluorescence intensity was linearly proportional to the concentration of adenosine, dopamine and 17β-estradiol with a limit of detection (LOD) of 101 nM, 19 nM and 37 nM, respectively. The experiments in fetal bovine serum were also carried out with good results. This universal method was rapid, label-free, low-cost, easy-operating and highly repeatable for the detection of adenosine, dopamine and 17β-estradiol. Qualitative detection by naked eyes was also available without complex instruments. It could also be extended to detect various analytes, such as metal ions, proteins and small molecules by using appropriate aptamers.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Hailiang Huang, Shuo Shi, Xing Gao, Ruru Gao, Ying Zhu, Xuewen Wu, Ruimin Zang, Tianming Yao,