Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7230918 | Biosensors and Bioelectronics | 2016 | 6 Pages |
Abstract
We report results of studies relating to the fabrication of a surface plasmon resonance (SPR)-based nucleic acid sensor for quantification of DNA sequence specific to chronic myelogeneous leukemia (CML). The SPR disk surface has been modified with octadecanethiol self-assembled monolayer followed by deposition of the tri-n-octylphosphine oxide capped cadmium selenide quantum dots (QD) Langmuir monolayer. The deposition is performed via Langmuir-Blodgett (LB) technique. For the sensor chip preparation, covalent immobilization of the thiol-terminated DNA probe sequence (pDNA) using displacement reaction is accomplished. This integrated SPR chip has been used to detect target complementary DNA concentration by monitoring the change in coupling angle via hybridization. It is revealed that this biosensor exhibits high sensitivity (0.7859Â m0Â pMâ1) towards complementary DNA and can be used to detect it in the concentration range, 180Â pM to 5Â pM with detection limit as 4.21Â pM. The results of kinetic studies yield the values of hybridization and dissociation rate constants as 9.6Ã104Â Mâ1Â sâ1 and 2.3Ã10â2Â sâ1, respectively, with the equilibrium constant for hybridization as 4.2Ã106Â Mâ1.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Aditya Sharma Ghrera, Manoj Kumar Pandey, Bansi Dhar Malhotra,