| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 7230977 | Biosensors and Bioelectronics | 2016 | 11 Pages |
Abstract
Analyte recognition and capture were confirmed with a parallel on fiber ELISA-like assay using, in pilot tests, the biotinylated protein and HRP-labeled streptavidin for its detection. Dose-dependent experiments showed that the detection is linearly dependent on concentration within the range between 0 and 4Â ng/mL, while antibody saturation occurs for higher protein levels. The system is characterized by a very high sensitivity and specificity allowing the ex-vivo detection of sub ng/ml concentrations of human Tg from needle washouts of fine-needle aspiration biopsies of thyroid nodule from different patients.
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Authors
Giuseppe Quero, Marco Consales, Renato Severino, Patrizio Vaiano, Alessandra Boniello, Annamaria Sandomenico, Menotti Ruvo, Anna Borriello, Laura Diodato, Simona Zuppolini, Michele Giordano, Immacolata Cristina Nettore, Claudia Mazzarella,