Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7231203 | Biosensors and Bioelectronics | 2016 | 6 Pages |
Abstract
The detection and speciation analysis of metal-ion is very important for environmental monitoring. A novel electrochemical biosensor for Nickel(II) detection based on a DNAzyme-CdSe nanocomposite was developed. We firstly hybridized with capture probe (DNA1) and sequentially with DNA (DNA2) on the gold electrode. Then CdSe QDs were incorporated the specific recognition of DNA2 by covalent assembling. Upon addition of nickel ion into the above system, the substrate strand of the immobilized DNAzyme was catalytically cleaved by target Ni2+, resulting in disassociation of the shorter DNA fragments containing CdSe QDs. The remaining CdSe QDs on the electrode surface detected by differential pulse anodic stripping voltammetry (DPASV). Under optimal conditions, the as-prepared sensor exhibited high sensitivity and fast response to Ni2+ with the linear range from 20Â nM to 0.2Â mM and a low detection limit of 6.67Â nM. The prepared biosensor also shows good stability and good reproducibility and high selectivity toward target Ni2+ against other metal ions because of highly specific Ni2+-dependent DNAzyme. Thus, our strategy has a good potential in the environment surveys.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Ying Yang, Zheng Yuan, Xing-Pei Liu, Qiao Liu, Chang-Jie Mao, He-Lin Niu, Bao-Kang Jin, Sheng-Yi Zhang,