Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7231384 | Biosensors and Bioelectronics | 2016 | 7 Pages |
Abstract
In this work, a one-pot method was designed to synthesize copper ions, cadmium ions, lead ions and zinc ions doped chitosan-poly(acrylic acid) nanospheres. Those nanospheres can not only produce independent electrochemical signals, but also react with glutaraldehyde (GA) to immobilize different labeled antibodies. Using the modified nanospheres as immunoprobes, a sandwich-type immunosensor was fabricated to simultaneous detection of four tumor markers (CEA, CA199, CA125 and CA242) of pancreatic cancer. This designed immunosensor exhibited good linear relationships in range from 0.1 to 100Â ng mLâ1 for CEA, 1 to 150Â UÂ mLâ1 for CA199, CA125 and CA242, corresponding detection limits 0.02Â ng mLâ1, 0.4Â UÂ mLâ1, 0.3Â UÂ mLâ1 and 0.4Â UÂ mLâ1, respectively. Meanwhile, the immunosensor was applied in analysis of clinical serum samples, whose results were well agreed with the enzyme-linked immunosorbent assay (ELISA), indicating that the proposed immunosensor gave a hope for the identification and validation of specific early cancer.
Related Topics
Physical Sciences and Engineering
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Authors
Qinfeng Rong, Feng Feng, Zhanfang Ma,