Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7231550 | Biosensors and Bioelectronics | 2015 | 32 Pages |
Abstract
It was reported that Proprotein convertase subtilisin/kexin type 6 (PCSK6) can promote the progression of rheumatoid arthritis to a higher aggressive status. In this work, a novel visible light induced photoelectrochemical (PEC) platform was designed to detect PCSK6 gene. ZnO@CdTe nanocable arrays/carboxylated g-C3N4 used as the PEC signal generator. Hexagonal ZnO nanorods grew on ITO electrode firstly. CdTe were then electrodeposited on the ZnO nanorods surface to enhance the photogenerated h+/eâ separation efficiency. Carboxylated g-C3N4 was utilized to improve h+/eâ separation efficiency and anchor the capture probes of PCSK6 gene by the covalent bonding effect. The 5â² and 3â² primers captured PCSK6 ssDNA by the specific recognition. The linear range was 10Â pg/mL to 20.0Â ng/mL with a detection limit of 2Â pg/mL.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Xuehui Pang, Jihong Pan, Picheng Gao, Youying Wang, Liguo Wang, Bin Du, Qin Wei,