Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7231727 | Biosensors and Bioelectronics | 2015 | 13 Pages |
Abstract
In this paper, we developed a simple, low-cost and sensitive DNA sequences detection biosensor based on a label-free molecular beacon (MB) whose DNA hairpin structure terminal has a guanine-rich sequence that can enhance fluorescence of silver nanoclusters (Ag NCs). Without hybridization between hairpin probe and target DNA, the Ag NCs presented bright fluorescence for the proximity of guanine-rich sequences (GRSs). After binding with target DNA, the hairpin shape was destroyed which results in a decrease of the Ag NCs fluorescence intensity. With this biosensor, we detected three disease-related genes that were the human immunodeficiency virus (HIV) gene, hepatitis B virus (HBV) gene and human T-lymphotropic virus type I (HTLV-I) gene. The detection limits based on S/N of 3 were 4.4Â nM, 6.8Â nM and 8.5Â nM for HIV gene, HBV gene and HTLV-I gene, respectively. Our sensor was also of high selectivity and could distinguish even one nucleotide mismatched target.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Qiao Cao, Ye Teng, Xuan Yang, Jin Wang, Erkang Wang,