Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7231794 | Biosensors and Bioelectronics | 2015 | 7 Pages |
Abstract
We present a direct, rapid and chemical-free detection method for hemoglobin concentration ([Hb]), based on photothermal angular light scattering. The iron oxides contained in hemoglobin molecules exhibit high absorption of 532-nm light and generate heat under the illumination of 532-nm light, which subsequently alters the refractive index of blood. We measured this photothermal change in refractive index by employing angular light scattering spectroscopy with the goal of quantifying [Hb] in blood samples. Highly sensitive [Hb] measurement of blood samples was performed by monitoring the shifts in angularly dispersed scattering patterns from the blood-loaded microcapillary tubes. Our system measured [Hb] over the range of 0.35-17.9Â g/dL with a detection limit of ~0.12Â g/dL. Our sensor was characterized by excellent correlation with a reference hematology analyzer (r>0.96), and yielded a precision of 0.63Â g/dL for a blood sample of 9.0Â g/dL.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Uihan Kim, Jaewoo Song, Donghak Lee, Suho Ryu, Soocheol Kim, Jaehyun Hwang, Chulmin Joo,