Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7231833 | Biosensors and Bioelectronics | 2015 | 7 Pages |
Abstract
Herein, an origami multiplexed enzyme-free electrochemical (EC) immunodevice is developed for the first time. Typically, ZnO nanorods (ZNRs) modified reduced graphene oxide (rGO)-paper electrode is used as a sensor platform, in which rGO improves the electronic transmission rate and ZNRs provide abundant sites for capture probes binding. Furthermore, by combining the large surface area of rGO and high catalytic activity of bovine serum protein (BSA)-stabilized silver nanoparticles (Ag@BSA) toward H2O2 reduction, rGO/Ag@BSA composites can be used as an excellent signal labels. The current signal is generated from the reduction of H2O2 and further amplified by a subsequent signal labels-promoted deposition of silver. Under optimal conditions, the proposed immunoassays exhibit excellent precision, high sensitivity and a wide linear range of 0.002-120 mIU mLâ1 for human chorionic gonadotropin, 0.001-110 ng mLâ1 for prostate-specific antigen, and 0.001-100 ng mLâ1 for carcinoembryonic antigen. The results for real sample analysis demonstrate that the newly constructed immunosensor arrays provide a simple and cost-effective method for clinical applications.
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Authors
Guoqiang Sun, Lina Zhang, Yan Zhang, Hongmei Yang, Chao Ma, Shenguang Ge, Mei Yan, Jinghua Yu, Xianrang Song,