Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7231842 | Biosensors and Bioelectronics | 2015 | 5 Pages |
Abstract
We describe here the construction of the DNA self-assembled molecular tweezers and the application of the tweezers for the monitoring of microRNA (miR-141) from human prostate cancer cells. The self-assembly formation of the DNA tweezers and the regulation of the tweezers upon alternative addition of the fuel miR-141 and the anti-fuel strands are characterized by native polyacrylamide gel electrophoresis. The addition of miR-141 to the DNA tweezers turns “off” the tweezers, while subsequent introduction of the anti-fuel strands switches the tweezers back to the “on” state, which verifies the regulatory ability of the tweezers. The miR-141-regulated DNA tweezers are concentration dependent and can be employed for sensitive detection of miR-141 down to 0.6Â pM. The DNA tweezers also show high selectivity toward the fuel strand and can be used to monitor miR-141 expression in cancer cells, which provides new opportunities for the application of the dynamic DNA devices in clinical diagnostics.
Related Topics
Physical Sciences and Engineering
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Analytical Chemistry
Authors
Xue Gong, Wenjiao Zhou, Daxiu Li, Yaqin Chai, Yun Xiang, Ruo Yuan,