Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7231889 | Biosensors and Bioelectronics | 2015 | 7 Pages |
Abstract
In this work, a novel polyamidoamine-norfloxacin (PAMAM-NFLX) complex and core-shell Pd-Au hexoctahedrons (Pd@Au HOHs) as enhancers are employed for development of a sensitive sandwich-type electrochemiluminescence (ECL) immunosensor to detect thyroid stimulating hormone (TSH). Here, norfloxacin (NFLX) is decorated abundantly on the surface of polyamidoamine (PAMAM) dendrimer via amide linkage to form PAMAM-NFLX complex. Thus, the resultant PAMAM-NFLX can serve as a novel co-reactant to efficiently amplify the ECL signal of peroxydisulfate-oxygen (S2O82â-O2) system. Pd@Au HOHs were used as nano-carriers to assemble detection antibody (Ab2) and the PAMAM-NFLX complex. Besides, it can further enhance the ECL signal by promoting the generation of intermediate free radical HO
- during the ECL reaction of S2O82â-O2 system. The proposed immunosensor shows high sensitivity and specificity, and responds linearly to the concentration of TSH from 0.05 to 20 μIU mLâ1 with a low detection limit of 0.02 μIU mLâ1 (S/N=3). Moreover, the immunosensor successfully achieves the detection of TSH in practical human blood serum with desirable results.
- during the ECL reaction of S2O82â-O2 system. The proposed immunosensor shows high sensitivity and specificity, and responds linearly to the concentration of TSH from 0.05 to 20 μIU mLâ1 with a low detection limit of 0.02 μIU mLâ1 (S/N=3). Moreover, the immunosensor successfully achieves the detection of TSH in practical human blood serum with desirable results.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Yuting Liu, Qiqi Zhang, Haijun Wang, Yali Yuan, Yaqin Chai, Ruo Yuan,