| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 7232202 | Biosensors and Bioelectronics | 2015 | 5 Pages |
Abstract
Electrochemical DNA (E-DNA) sensor is an important tool for detecting DNA biomarker. In this work, we have demonstrated a novel strategy of E-DNA sensor based on DNA tetrahedral nanostructures for the sensitive detection of target DNA. In our design, thiol and biotin modified DNA tetrahedral nanostructures were used as capture and report probes respectively. The biotin-tagged three dimensional DNA tetrahedral nanostructures were employed for efficient signal amplification by capturing multiple catalytic enzymes. Such improved E-DNA sensor can sensitively detect DNA target as low as 1Â fM with excellent differentiation ability for even single mismatch. And a mean recovery rate of 90.57% in DNA solution extracted from human serum was obtained. We have also compared this new method of attaching catalytic enzymes with the other two typical methods: One is through biotinylated single-stranded DNA (SSDNA) and the other is through gold nanoparticles (GNPs). Results indicated that the RTSPs-based enzyme amplification system showed much better performance than the other two systems.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Dongdong Zeng, Huan Zhang, Dan Zhu, Jiang Li, Lili San, Zehua Wang, Chenguang Wang, Yunsheng Wang, Lihua Wang, Xiaolei Zuo, Xianqiang Mi,