| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 7232329 | Biosensors and Bioelectronics | 2015 | 5 Pages |
Abstract
Lead ion (Pb2+, a heavy metal ion existed every aspect of life) is one of the most important hazards for environment and human health. Herein we design an in-situ amplified electronic monitoring system for sensitive determination of Pb2+ on Pb2+-specific DNAzyme-modified sensing interface. The assay consists of target-triggered cleavage of Pb2+-specific DNAzyme, initiator strand-induced DNA hybridization chain reaction, formation of hemin/G-quadruplex -based DNAzyme and its automatically catalyzed polymerization of aniline monomer along the double-stranded DNA. Experimental results indicated that the catalytic currents of the as-produced polyaniline were linearly dependent on target Pb2+ concentrations from 0.05 to 50Â nM with a detection limit (LOD) of 32Â pM. Such a synergistic effect of hybridization chain reaction with DNAzyme-catalyzed polymerization provided a universal platform for sensitive screening of target Pb2+, thereby holding great promise for application in practice.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Bing Zhang, Jinfeng Chen, Bingqian Liu, Dianping Tang,