Article ID Journal Published Year Pages File Type
7232362 Biosensors and Bioelectronics 2015 7 Pages PDF
Abstract
Monitoring of reactive oxygen species like superoxide anion (O2∙−) turns to be of increasing significance considering their potential damages to organism. In the present work, we fabricated a novel O2∙− electrochemical sensor through immobilizing superoxide dismutase (SOD) onto a Pt-Pd/MWCNTs hybrid modified electrode surface. The Pt-Pd/MWCNTs hybrid was synthesized via a facile one-step alcohol-reduction process, and well characterized by transmission electron microscopy, X-ray photoelectron spectroscopy and X-ray diffraction. The immobilization of SOD was accomplished using a simple drop-casting method, and the performance of the assembled enzyme-based sensor for O2∙− detection was systematically investigated by several electrochemcial techniques. Thanks to the specific biocatalysis of SOD towards O2∙− and the Pt-Pd/MWCNTs - promoted fast electron transfer at the fabricated interface, the developed biosensor exhibits a fast, selective and linear amperometric response upon O2∙− in the concentration scope of 40-1550 μM (R2=0.9941), with a sensitivity of 0.601 mA cm−2 mM−1 and a detection limit of 0.71 μM (S/N=3). In addition, the favorable biocompatibility of this electrode interface endows the prepared biosensor with excellent long-term stability (a sensitivity loss of only 3% over a period of 30 days). It is promising that the proposed sensor will be utilized as an effective tool to quantitatively monitor the dynamic changes of O2∙− in biological systems.
Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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