| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 7232418 | Biosensors and Bioelectronics | 2015 | 5 Pages |
Abstract
A novel optical detection system consisting of combination of uricase/HRP-CdS quantum dots (QDs) for the determination of uric acid in urine sample is described. The QDs was used as an indicator to reveal fluorescence property of the system resulting from enzymatic reaction of uricase and HRP (horseradish peroxidase), which is involved in oxidizing uric acid to allaintoin and hydrogen peroxide. The hydrogen peroxide produced was able to quench the QDs fluorescence, which was proportional to uric acid concentration. The system demonstrated sufficient activity of uricase and HRP at a ratio of 5U:5U and pH 7.0. The linearity of the system toward uric acid was in the concentration range of 125-1000 µM with detection limit of 125 µM.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Nur Ellina Azmi, Noor Izaanin Ramli, Jaafar Abdullah, Mohammad Azmi Abdul Hamid, Hamidah Sidek, Samsulida Abd Rahman, Nurhayati Ariffin, Nor Azah Yusof,