Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7232502 | Biosensors and Bioelectronics | 2015 | 7 Pages |
Abstract
MicroRNAs (miRNAs) play vital regulatory roles in cancer development and a variety of diseases, which make them become promising biomarkers. Here, a simple electrochemical biosensor was developed for highly sensitive and specific detection of target miRNA using mismatched catalytic hairpin assembly (CHA). The target miRNA triggered the toehold strand displacement assembly of two hairpin substrates, which led to the cyclic reuse of the target miRNA and the CHA products. Compared with the traditional CHA, mismatched CHA could decrease the nonspecific CHA products, which reduced the background signal significantly. Under the optimal experimental conditions and using differential pulse voltammetry, the established biosensor could detect target miRNA down to 0.6Â pM (S/N=3) with a linear range from 1Â pM to 25Â nM, and discriminate target miRNA from mismatched miRNA with a high selectivity. It was also applied to the determination of miRNA spiked into human total RNA samples. Thus, this biosensing strategy might become a potential alternative tool for detection of miRNA in biomedical research and early clinical diagnosis.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Ye Zhang, Yurong Yan, Wenhong Chen, Wei Cheng, Shengqiang Li, Xiaojuan Ding, Dandan Li, Hong Wang, Huangxian Ju, Shijia Ding,