Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7232570 | Biosensors and Bioelectronics | 2015 | 5 Pages |
Abstract
An ultrasensitive electrochemical DNA biosensor for human papillomavirus (HPV) detection was developed by electrochemical impedance spectroscopy and differential pulse voltammetry. A capture probe was immobilized on a glassy carbon electrode modified with graphene/Au nanorod/polythionine (G/Au NR/PT). Two auxiliary probes were designed and used to long-range self-assemble DNA nanostructure. The target DNA can connect DNA structure to the capture probe on the electrode surface. [Ru(phen)3]2+ was selected as a redox indicator for amplifying electrochemical signal significantly. Enhanced sensitivity was obtained through combining the excellent electric conductivity of G/Au NR/PT architecture and the long-range self-assembly DNA nanostructure with the multi-signal amplification. The DNA biosensor displayed excellent performance for HPV DNA detection over the range from 1.0Ã10-13 to 1.0Ã10-10Â mol/L with a detection limit of 4.03Ã10-14Â mol/L. Furthermore, the proposed method can also be used for the detection of HPV DNA in human serum samples and provides a potential application of DNA detection in clinic research.
Related Topics
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Authors
Huayu Huang, Wanqiao Bai, Changxun Dong, Rui Guo, Zhihua Liu,