Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7232634 | Biosensors and Bioelectronics | 2015 | 6 Pages |
Abstract
This study presents a novel method for CD4 testing based on one-shot large-field imaging. The large-field imaging system was fabricated by a microcavity array and a two-dimensional (2D) photosensor within the desk-top-sized instrument. The microcavity array was employed to separate leukocytes from whole blood based on differences in the size of leukocytes and other blood cells. The large-field imaging system with lower side irradiation enabled acquisition of cell signatures with high signal-to-noise ratio, because the metallic substrate of the microcavity array obstructed excessive excitation light. In this setting, dual-color imaging of CD4+ and CD8+ T cells was achieved within the entire image area (64Â mm2) in 2Â s. The practical performance of the large-field imaging system was demonstrated by determining the CD4/CD8 ratio in a few microliter of control whole blood as small as those obtained by a finger prick. The CD4/CD8 ratios measured using the large-field imaging system correlated well with those measured by microscopic analysis. These results indicate that our proposed system provides a simple and rapid CD4 testing for the application of HIV/AIDS treatment.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Tatsuya Saeki, Yuriko Sugamura, Masahito Hosokawa, Tomoko Yoshino, Tae-kyu Lim, Manabu Harada, Tadashi Matsunaga, Tsuyoshi Tanaka,