Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7232704 | Biosensors and Bioelectronics | 2015 | 5 Pages |
Abstract
Methicillin-resistant Staphylococcus aureus (MRSA) is one of the most common causes of hospital-acquired infections. To prevent epidemics, a quick and simple detection method is required. In this study, we developed a novel electrochemical DNA detection method that does not rely upon polymerase chain reaction (PCR) and may be used in point-of-care facilities. The electrochemical DNA sensing system presented here is based on the chronoamperometric detection of ferrocene-labeled probes that were conjugated to gold nanoparticles (AuNPs). This DNA sensor system employed magnetic nanoparticle (MNP)-modified probes allowing easy sample DNA recovery. AuNP nanoparticles with ferrocene-labeled probes enabled the generation of an electric signal, and MNP/DNA/AuNP conjugates were formed by hybridization. Following hybridization, the MNP/DNA/AuNP hybridization complex is magnetically separated, and electrochemical current responses could be obtained because of the AuNP-ferrocene complexes. To construct a highly sensitive system, dye-linked l-proline dehydrogenase (l-proDH) was employed to amplify current responses following a catalytic reaction with l-proline. Rapid catalytic reaction of l-proDH and substrate was able to amplify the oxidation of ferrocene. Target DNA from MRSA could be quantified over a range of 10-166Â pM, and this sensing system could also distinguish MRSA from S. aureus.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Kazuya Watanabe, Noriaki Kuwata, Hiroaki Sakamoto, Yoshihisa Amano, Takenori Satomura, Shin-ichiro Suye,