Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7232826 | Biosensors and Bioelectronics | 2015 | 7 Pages |
Abstract
Detection of Bacillus anthracis in the field, whether as a natural infection or as a biothreat remains challenging. Here we have developed a new lateral-flow immunochromatographic assay (LFIA) for B. anthracis spore detection based on the fact that conjugates of B. anthracis spores and super-paramagnetic particles labeled with antibodies will block the pores of chromatographic strips and form retention lines on the strips, instead of the conventionally reported test lines and control lines in classic LFIA. As a result, this new LFIA can simultaneously realize optical, magnetic and naked-eye detection by analyzing signals from the retention lines. As few as 500-700 pure B. anthracis spores can be recognized with CV values less than 8.31% within 5Â min of chromatography and a total time of 20Â min. For powdery sample tests, this LFIA can endure interference from 25% (w/v) milk, 10% (w/v) baking soda and 10% (w/v) starch without any sample pre-treatment, and has a corresponding detection limit of 6Ã104 spores/g milk powder, 2Ã105 spores/g starch and 5Ã105 spores/g baking soda. Compared with existing methods, this new approach is very competitive in terms of sensitivity, specificity, cost and ease of operation. This proof-of-concept study can also be extended for detection of many other large-sized analytes.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Dian-Bing Wang, Bo Tian, Zhi-Ping Zhang, Xu-Ying Wang, Joy Fleming, Li-Jun Bi, Rui-Fu Yang, Xian-En Zhang,