Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7232868 | Biosensors and Bioelectronics | 2015 | 6 Pages |
Abstract
The discovery and application of noble metal nanoclusters have received considerable attention. In this paper, we reported that apoferritin paired gold clusters (Au-Ft) could efficiently catalyze oxidation of 3.3â²,5.5â²-tetramethylbenzidine (TMB) by H2O2 to produce a blue color reaction. Compared with natural enzyme, Au-Ft exhibited higher activity near acidic pH and could be used over a wide range of temperatures. Apoferritin nanocage enhanced the reaction activity of substrate TMB by H2O2. The reaction catalyzed by Au-Ft was found to follow a typical Michaelis-Menten kinetics. The kinetic parameters exhibited a lower Km value (0.097Â mM) and a higher Kcat value (5.8Ã104Â sâ1) for TMB than that of horse radish peroxidase (HRP). Base on these findings, Au-Ft, acting as a peroxidase mimetic, performed enzymatic spectrophotometric analysis of glucose. This system exhibited acceptable reproducibility and high selectivity in biosening, suggesting that it could have promising applications in the future.
Related Topics
Physical Sciences and Engineering
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Authors
Xin Jiang, Cuiji Sun, Yi Guo, Guangjun Nie, Li Xu,