Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7232909 | Biosensors and Bioelectronics | 2015 | 5 Pages |
Abstract
Here we describe a sensitive assay for thrombin by using a high binding-affinity cyclic peptide against thrombin as affinity ligand. The cyclic peptide is immobilized on the magnetic beads or microplates to selectively capture thrombin. The enriched thrombin then catalyzes the cleavage of a substrate of thrombin to a detectable product. The detection of thrombin is finally achieved by measuring the generated product. This assay enables the detection of thrombin at tens fM in 100 µL of sample solution when fluorogenic substrate was applied, while detection limits reached pM level when chromogenic substrate was used. Thrombin in plasma sample can be detected with this assay. This cyclic peptide affinity ligand shows potentials for thrombin analysis in other detection formats.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Qiang Zhao, Jie Gao,