Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7233080 | Biosensors and Bioelectronics | 2014 | 7 Pages |
Abstract
An evanescent wave fiber optic sensor for detection of Escherichia coli (E. coli) outer membranes proteins (EcOMPs) using long period gratings (LPGs) as a refractometric platform is presented. The sensing probes were attained by the functionalization of LPGs inscribed in single mode fiber using two different methods of immobilization; electrostatic assembly and covalent binding. The resulting label-free configuration enabled the specific recognition of EcOMPs in water by monitoring the resonance wavelength shift due to refractive index changes induced by binding events. The sensors displayed linear responses in the range of 0.1 nM to 10 nM EcOMPs with sensitivities of â0.1563±0.005 nm decadeâ1 [EcOMP, M] (electrostatic method) and â0.1597±0.004 nm decadeâ1 [EcOMP, M] (covalent method). The devices could be regenerated (under low pH conditions) with a deviation less than 0.1% for at least three subsequent detection events. The sensors were also applied to spiked environmental water samples.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
R.B. Queirós, C. Gouveia, J.R.A. Fernandes, P.A.S. Jorge,