| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 7233242 | Biosensors and Bioelectronics | 2014 | 5 Pages |
Abstract
A label-free method for the measurement of the activity of HIV-1 protease is developed by real-time monitoring of the cleavage of a peptide substrate by HIV-1 protease in a nanopore. The method is rapid and sensitive: picomolar concentrations of HIV-1 protease could be detected in ~10Â min. Simulated clinical samples are analyzed, and the activity of HIV-1 protease could be accurately detected. Our developed nanopore sensor design strategy should find useful applications in the development of stochastic sensors for other proteases of medical, pharmaceutical, and biological importance.
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Authors
Liang Wang, Yujing Han, Shuo Zhou, Xiyun Guan,