Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7233358 | Biosensors and Bioelectronics | 2014 | 19 Pages |
Abstract
A new biosensing platform based on electrospun carbon nanotubes nanofibers (CNTs@PNFs) composite, which enabled strong electrochemiluminescent emission of peroxydisulfate, was firstly developed for immunoassay with favorable analytical performances, and then was utilized to evaluate the interaction between antibody and antigen in vitro. Moreover, the obvious ECL image of peroxydisulfate on the prepared sensing platform was firstly recorded in this report. In order to expand the application of peroxydisulfate ECL, the specific recognization biomolecules, α-fetoprotein (AFP) antibody was bound to the functionalized film via electrostatic interaction for fabricating label-free ECL immunosensor to detect α-AFP. Based on the ECL change resulting from the specific immunoreaction between antigen and antibody, the quantitative analysis for AFP with wide dynamic response in the range from 0.1 pg mLâ1 to 160 ng mLâ1 was realized. And the limit of detection was estimated to be 0.09 pg mLâ1. Therefore, the flexible sensing platform not only acted as the sensitized sensing element, but also offered a suitable carrier for immobilization of biological recognition elements with low-toxicity and eco-friendliness, which opened a promising approach to developing further electrospun nanofiber based amplified ECL biosensor with favorable analytical performances.
Related Topics
Physical Sciences and Engineering
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Authors
Hong Dai, Guifang Xu, Shupei Zhang, Lingshan Gong, Xiuhua Li, Caiping Yang, Yanyu Lin, Jinghua Chen, Guonan Chen,