Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7233510 | Biosensors and Bioelectronics | 2014 | 8 Pages |
Abstract
Zinc oxide (ZnO) nanostructures possessing flower-like morphology have been synthesised onto platinized silicon substrate by simple and economical hydrothermal method. The interaction of physically immobilized single stranded thiolated DNA (ss th-DNA) probe of N. meningitides onto the nanostructured ZnO (ZNF) matrix surface have been investigated using cyclic voltammetry (CV) and electrochemical impeadance spectroscopy (EIS). The electrochemical sensing response behaviour of the DNA bioelectrode (ss th-DNA/ZNF/Pt/Si) has been studied by both differential pulse voltammetric (DPV) as well as impedimetric techniques. The fabricated DNA biosensor can quantify wide range of the complementary target ss th-DNA in the range 5-240 ng μlâ1 with good linearity (R=0.98), high sensitivity (168.64 μA ngâ1 μl cmâ2) and low detection limit of about 5 ng μlâ1. Results emphasise that the fabricated flower-like ZnO nanostructures offer a useful platform for the immobilization of DNA molecules and could be exploited for efficient detection of complementary target single stranded DNA corresponding to N. meningitides.
Related Topics
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Authors
Manvi Tak, Vinay Gupta, Monika Tomar,