Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7233528 | Biosensors and Bioelectronics | 2014 | 7 Pages |
Abstract
A biosensor has been developed with a photonic crystal structure used in a total-internal-reflection (PC-TIR) configuration for label-free detection of a cardiac biomarker: Troponin I (cTnI). In contrast to a conventional optical microcavity that has a closed structure with its cavity layer sandwiched between two high-reflection surfaces, the PC-TIR configuration creates a unique open microcavity, which allows its cavity layer (sensing layer) to be easily functionalized and directly exposed to analyte molecules for bioassays. In this study, a PC-TIR sensor has been used for the label-free measurements of cardiac biomarkers by monitoring the changes in the resonant condition of the cavity due to biomolecular binding processes. Antibodies against cTnI are immobilized on the sensor surface for specific detection of cTnI with a wide range of concentrations. Detection limit of cTnI with a concentration as low as 0.1 ng mLâ1 has been achieved.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Bailin Zhang, Andres W. Morales, Ralph Peterson, Liang Tang, Jing Yong Ye,