Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7233573 | Biosensors and Bioelectronics | 2014 | 8 Pages |
Abstract
Uniform and highly dispersed gold-silicon carbide (Au@SiC) nanocomposites were prepared via simple way and used for fabrication of label-free electrochemical immunosensor for sensitive detection of human chorionic gonadotrophin (hCG). Using Au@SiC as electrode material and using ferricyanide as mediator, the proposed immunosensor provides a simple and economic method with higher sensitivity and a wider concentration range for detection of hCG. Under the optimal condition, the approach provided a good linear response range from 0.1 to 5Â IU/L and 5 to 1000Â IU/L with a low detection limit of 0.042Â IU/L. The immunosensor showed good selectivity, acceptable stability and reproducibility. Satisfactory results were obtained for determination of hCG in human serum samples. The proposed method provides a promising platform of clinical immunoassay for other biomolecules. In addition, the bio-functionalization of SiC combined with other nanomaterials will provide promising approach for electrochemical sensing and biosensing platform.
Related Topics
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Analytical Chemistry
Authors
Long Yang, Hui Zhao, Shuangmei Fan, Shuangsheng Deng, Qi Lv, Jie Lin, Can-Peng Li,