Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7233583 | Biosensors and Bioelectronics | 2014 | 7 Pages |
Abstract
In this article, for the first time, a novel, high-yield and template-free method for the synthesis of Ag nanoparticle decorated thionine/infinite coordination polymer (AgNP/THI/ICP) fibres is proposed. The thionine can be adsorbed to the AgNP/THI/ICP fibres by Ï-conjugation and act as the redox probe. The AgNP/THI/ICP fibres not only favor the immobilization of antibody but also facilitate the electron transfer. It is found that the AgNP/THI/ICP fibres can be designed to act as a sensitive label-free electrochemical immunosensor for carcinoembryonic antigen (CEA) determination. Under the optimized conditions, the linear range of the proposed immunosensor is estimated to be from 50Â fg/mL to 100Â ng/mL and the detection limit is estimated to be 0.5Â fg/mL at a signal-to-noise ratio of 3, respectively. The prepared immunosensor for detection of CEA shows high sensitivity, reproducibility and stability. Our study demonstrates that the proposed immunosensor has also been used to determine CEA successfully in diluted blood samples.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Wenbo Lu, Xiaowei Cao, Lin Tao, Juan Ge, Jian Dong, Weiping Qian,