ON THE MODELING OF PARAFFIN THROUGH RAMAN SPECTROSCOPY

Raman spectroscopy is employed to record spectra which highlight vibrational information of biological structures. These spectra provide useful information about molecular composition of a tissue. However, the tissues are usually embedded into paraffin for a preservation purpose. The Independent Component Analysis (ICA) technique can be used to numerically dewax Raman spectra and to extract the specific information of the tissue. We show in this paper that the paraffin must be modeled by a three-source model in order to succeed. This linear model, confirmed also by analyzing paraffin blocks exclusively, should be preferred instead of commonly used one-source model.