Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7233645 | Biosensors and Bioelectronics | 2014 | 6 Pages |
Abstract
In this study, we report a strategy of chemiluminescence resonance energy transfer (CRET) using graphene as an efficient long-range energy acceptor. Magnetic nanoparticles were also used in CRET for simple magnetic separation and immobilization of horseradish peroxidase (HRP)-labeled anti-HCG antibody. In the design of CRET system, the sandwich-type immunocomplex was formed between human chorionic gonadotropin (HCG, antigen) and two different antibodies bridged the magnetic nanoparticles and graphene (acceptors), which led to the occurrence of CRET from chemiluminescence light source to graphene. After optimizing the experimental conditions, the quenching of chemiluminescence signal depended linearly on the concentration of HCG in the range of 0.1Â mIUÂ mLâ1-10Â mIUÂ mLâ1 and the detection limit was 0.06Â mIUÂ mLâ1. The proposed method was successfully applied for the determination of HCG levels in saliva and serum samples, and the results were in good agreement with the plate ELISA with colorimetric detection. It could also be developed for detection of other antigen-antibody immune complexes by using the corresponding antigens and respective antibodies.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Jiuqian Lei, Tao Jing, Tingting Zhou, Yusun Zhou, Wei Wu, Surong Mei, Yikai Zhou,