Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7233684 | Biosensors and Bioelectronics | 2014 | 7 Pages |
Abstract
Blood safety is a global health goal. In developed countries, bacterial contamination of platelet concentrates is the highest infectious risk in transfusion despite the current preventive strategies. We aimed to develop a conductometric biosensor for the generic, rapid and sensitive detection of Gram-negative bacteria. Our strategy is based on immunosensors: addressable magnetic nanoparticles coupled with anti-LPS antibodies were used for the generic capture of Gram-negative bacteria. Bacterial capture was characterized by impedancemetric and microscopic measurements. The results obtained with conductometric measurements allowed real-time, sensitive detection of Escherichia coli or Serratia marcescens cultures from 1 to 103Â CFUÂ mLâ1. The ability of the immunosensor to detect Gram negative bacteria was also tested on clinically relevant strains. The conductometric immunosensor allowed the direct detection of 10-103Â CFUÂ mLâ1 of Pseudomonas aeruginosa and Acinetobacter baumannii strains that were undetectable using standard immunoblot methods. Results showed that the conductometric response was not inhibited in 1% serum.
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Authors
Sarra El Ichi, Fanny Leon, Ludivine Vossier, Helene Marchandin, Abdelhamid Errachid, Joliette Coste, Nicole Jaffrezic-Renault, Chantal Fournier-Wirth,