Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7233706 | Biosensors and Bioelectronics | 2014 | 7 Pages |
Abstract
This manuscript describes the on-chip quantification of Tumour Necrosis Factor-alpha (TNF-α) within the clinical relevant range of 5-100 pg/mL in human plasma by means of a magnetic bead-based proximity ligation assay (PLA). The TNF-α-DNA template complex was amplified by qPCR on a microfluidic setup developed in-house.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Vanessa Castro-López, Jorge Elizalde, Marcin Pacek, Elizabeth Hijona, Luis Bujanda,