Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7233756 | Biosensors and Bioelectronics | 2014 | 7 Pages |
Abstract
We report a DNA-gold nanoparticle (DNA-GNP) based lateral flow nucleic acid biosensor for visual detection of microRNA (miRNA)-215 in aqueous solutions and biological samples with low-cost and short analysis time. Sandwich-type hybridization reactions among GNP-labeled DNA probe, miRNA-215 and biotin-modified DNA probes were performed on the lateral flow device. The accumulation of GNPs on the test zone of the biosensor enables the visual detection of miRNA-215. After systematic optimization, the biosensor was able to detect a minimum concentration of 60Â pM miRNA-215. The biosensor was applied to detect miRNA-215 from A549 cell lysate directly without complex sample treatment, and the detection limit of 0.148 million cells was obtained. This study provides a simple, rapid, specific and low-cost approach for miRNA detection in aqueous solutions and biological samples, showing great promise for clinical application and biomedical diagnosis in some malignant diseases.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Xuefei Gao, Hui Xu, Meenu Baloda, Anant S. Gurung, Li-Ping Xu, Tao Wang, Xueji Zhang, Guodong Liu,